anthropology

stone-tools-may-hint-at-ancestors-of-homo-floresiensis

Stone tools may hint at ancestors of Homo floresiensis

Some stone tools found near a river on the Indonesian island of Sulawesi suggest that the first hominins had reached the islands by at least 1.04 million years ago. That’s around the same time that the ancestors of the infamously diminutive “Hobbits” may have reached the island of Flores.

Archaeologist Budianto Hakim of Indonesia’s National Research and Innovation Agency and his colleagues were the ones who recently unearthed the tools from a site on Sulawesi. Although a handful of stone flakes from that island don’t tell us who the ancestors of the small species were or how they reached remote islands like Flores and Luzon, the tools are one more piece in the puzzle. And this handful of stone flakes may eventually play a role in helping us understand how other hominin species conquered most of the world long before we came along. 

Crossing the ocean a million years ago

Sometimes the deep past leaves the smallest traces. At the Calio site, a sandstone outcrop in what’s now a cornfield outside the village of Ujung in southern Sulawesi, people left behind just a handful of sharp stone flakes roughly a million years ago. There are seven of them, ranging from 22 to 60 millimeters long, and they’re scratched, worn, and chipped from tumbling around at the bottom of a river. But it’s still clear that they were once shaped by skilled human—or at least human-like—hands that used hard stones as hammers to make sharp-edged chert flakes for cutting and scraping.

The oldest of these tools is likely to be between 1.04 and 1.48 million years old. Hakim and his colleagues dated teeth from a wild pig to around 1.26 million years ago. They were part of a jawbone archaeologists unearthed from a layer just above the oldest flake. Throw in some statistical modeling, and you get the range of likely dates for the stone flake buried in the deepest layer of soil.

Even the younger end of that estimate would make these tools the oldest evidence yet of hominins (of any species) in the islands of Indonesia and the Philippines. This area, sometimes called Wallacea, lies between the continents of Asia and Australia, separated from both by wide channels of deep ocean.

“But the Calio site has yet to yield any hominin fossils,” said Brumm, “so while we now know there were tool-makers on Sulawesi a million years ago, their identity remains a mystery.” But they may be related to the Hobbits, a short-statured group of hominins who lived hundreds of kilometers away on the island of Flores until around 50,000 years ago.

“The discovery of Early Pleistocene artifacts at Calio suggests that Sulawesi was populated by hominins at around the same time as Flores, if not earlier,” wrote Hakim and his colleagues in their recent paper. 

The Flores connection

The islands that now make up Indonesia and the Philippines have been a hominin hotspot for at least a million years. Our species wandered onto the scene sometime between 63,000 and 73,000 years ago, but at least one other hominin species had already been there for at least a million years. We’re just not sure exactly who they were, when they arrived, or how.

“Precisely when hominins first crossed to Sulawesi remains an open question, as does the taxonomic affinity of the colonizing population,” the authors note. 

map of Wallacean islands

This map shows the islands of Wallacea. The large one just east of Java is Sulawesi. Credit: Darren O’Connell

That’s why the handful of stone tools the team recently unearthed at Calio matter: They’re another piece of that puzzle, albeit a small one. Every slightly older date is one step closer to the first hominin tools, bones, or footprints in these islands, and another pin on the map of who was where and when.

And that map is accumulating quite a lot of pins, representing an ever-increasing number of species. Once the first hominins made it across the Makassar Strait, they found themselves in isolated groups on islands cut off from the mainland—and each other—so the hominin family tree started branching very quickly. On at least two islands, Flores and Luzon, those original hominin settlers eventually gave rise to local species, Homo floresiensis and Homo luzonensis. And University of Wollongong paleoanthropologist Richard Roberts, a co-discoverer of Homo floresiensis, thinks there are probably more isolated island hominin species.

In 2019, when Homo luzonensis was first described, Roberts told Ars, “These new fossils, and the assignation of them to a new species (Homo luzonensis), fulfills one of the predictions Mike Morwood and others (myself included) made when we first reported (15 years ago!) the discovery of Homo floresiensis: that other unknown species of hominins would be found in the islands of Southeast Asia.”

Both Homo floresiensis (the original “Hobbits”) and Homo luzonensis were short, clocking in at just over a meter tall. Their bones and teeth are different enough from each other to set them apart as a unique species, but they have enough in common that they probably share a common ancestor—one they don’t share with us. They’re more like our distant cousins, and the islands of Wallacea may have been home to many other such cousins, if Roberts and his colleagues are correct. 

Complicated family history

But who was the common ancestor of all these hominin cousins? That’s where things get complicated (as if they weren’t already). Most paleoanthropologists lean toward Homo erectus, but there’s a chance—along with some tantalizing hints, and no direct evidence—that much more ancient human relatives called Australopithecines may have made the journey a million (or two) years before Homo erectus.

Finger and toe bones from Homo luzonensis are curved, as if they spent as much of their lives climbing trees as walking. That’s more like Australopithecines than any member of our genus Homo. But their teeth are smaller and shaped more like ours. Anthropologists call this mix of features a mosaic, and it can make it tough to figure out how hominin species are related. That’s part of why the question of when the ancestors of the Hobbits arrived on their respective islands is so important.

Illusstrated chart of bones and teeth from three hominins

Compare the teeth and phalanx of Homo luzonensis to those of Homo sapiens (right) and Australopithecus afarensis (left). Credit: Tocheri 2019

We don’t know the answer yet, but we do know that someone was making stone tools on Flores by 1.02 million years ago. Those toolmakers may have been Homo erectus, Australopithecines, or something already recognizable as tiny Homo floresiensis. The Hobbits (or their ancestors) were distinctly “Hobbity” by around 700,000 years ago; fossil teeth and bones from a handful of hominins at a site called Mata Menge make that clear. The Hobbits discovered at Liang Bua Cave on Flores date to somewhere between 50,000 and 100,000 years ago.

Meanwhile, 2,800 kilometers away on the island of Luzon, the oldest stone tools, along with their obvious cut marks left behind on animal bones, date back to 700,000 years ago. That’s as old as the Mata Menge Hobbits on Flores. The oldest Homo luzonensis fossils are between 50,000 and 67,000 years old. It’s entirely possible that older evidence, of the island’s original settlers and of Homo luzonensis, may eventually be found, but until then, we’re left with a lot of blank space and a lot of questions.

And now we know that the oldest traces of hominin presence on Sulawesi is at least 1.04 million years old. But might Sulawesi have its own diminutive hominins?

So are there more Hobbits out there?

“Sulawesi is a wild card—it’s like a mini-continent in itself,” said Brumm. “If hominins were cut off on this huge and ecologically rich island for a million years, would they have undergone the same evolutionary changes as the Flores hobbits? Or would something totally different have happened?”

Reconstruction of Homo floresiensis by Atelier Elisabeth Daynes. Credit: Kinez Riza

A phenomenon called island dwarfism played a role in Homo floresiensis‘ evolution; species that live in relative isolation on small islands tend to evolve into either much larger or much smaller versions of their ancestors (which is why the Hobbits shared their island home with pygmy elephants and giant moas). But how small does an island need to be before island dwarfism kicks in? Sulawesi is about 12 times as large as Flores, for example. So what might the descendants of the Calio toolmakers have looked like by 100,000 years ago?

That’s something that we’ll only know if archaeologists on Sulawesi, like Hakim and his team, find fossil remains of those hominins.

Seafarers or tsunami survivors?

Understanding exactly when hominins first set foot on the island of Sulawesi might eventually help us figure out how they got there. These islands are thousands of kilometers from the Southeast Asian mainland and from each other, so getting there would have meant crossing vast stretches of deep, open ocean.

Archaeologists haven’t found any evidence that anyone who came before our species built boats or rafts, although those watercraft would have been made of materials that tend to decay pretty quickly, so even scraps of ancient wood and rope are extremely rare and lucky finds. But some ancient hominins did have a decent grasp of all the basic skills they’d need for at least a simple raft: woodworking and rope-making. 

Another possibility is that hominins living on the coast of mainland Southeast Asia could have been swept out to sea by a tsunami, and some of them could have been lucky enough to survive the misadventure and wash ashore someplace like Sulawesi, Flores, or Luzon (RIP to any others). But for that scenario to work, enough hominins would have had to reach each island to create a lasting population, and it probably had to happen more than once to end up with hominin groups on at least three distant islands.

Either way, it’s no small feat, even for a Hobbit with small feet.

Nature, 2025 DOI: 10.1038/s41586-025-09348-6 (About DOIs).

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Fermented meat with a side of maggots: A new look at the Neanderthal diet

Traditionally, Indigenous peoples almost universally viewed thoroughly putrefied, maggot-infested animal foods as highly desirable fare, not starvation rations. In fact, many such peoples routinely and often intentionally allowed animal foods to decompose to the point where they were crawling with maggots, in some cases even beginning to liquefy.

This rotting food would inevitably emit a stench so overpowering that early European explorers, fur trappers, and missionaries were sickened by it. Yet Indigenous peoples viewed such foods as good to eat, even a delicacy. When asked how they could tolerate the nauseating stench, they simply responded, “We don’t eat the smell.”

Neanderthals’ cultural practices, similar to those of Indigenous peoples, might be the answer to the mystery of their high δ¹⁵N values. Ancient hominins were butchering, storing, preserving, cooking, and cultivating a variety of items. All these practices enriched their paleo menu with foods in forms that nonhominin carnivores do not consume. Research shows that δ¹⁵N values are higher for cooked foods, putrid muscle tissue from terrestrial and aquatic species, and, with our study, for fly larvae feeding on decaying tissue.

The high δ¹⁵N values of maggots associated with putrid animal foods help explain how Neanderthals could have included plenty of other nutritious foods beyond only meat while still registering δ¹⁵N values we’re used to seeing in hypercarnivores.

We suspect the high δ¹⁵N values seen in Neanderthals reflect routine consumption of fatty animal tissues and fermented stomach contents, much of it in a semi-putrid or putrid state, together with the inevitable bonus of both living and dead ¹⁵N-enriched maggots.

What still isn’t known

Fly larvae are a fat-rich, nutrient-dense, ubiquitous, and easily procured insect resource, and both Neanderthals and early Homo sapiens, much like recent foragers, would have benefited from taking full advantage of them. But we cannot say that maggots alone explain why Neanderthals have such high δ¹⁵N values in their remains.

Several questions about this ancient diet remain unanswered. How many maggots would someone need to consume to account for an increase in δ¹⁵N values above the expected values due to meat eating alone? How do the nutritional benefits of consuming maggots change the longer a food item is stored? More experimental studies on changes in δ¹⁵N values of foods processed, stored, and cooked following Indigenous traditional practices can help us better understand the dietary practices of our ancient relatives.

Melanie Beasley is assistant professor of anthropology at Purdue University.

This article is republished from The Conversation under a Creative Commons license. Read the original article.

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testing-ancient-paleolithic-migration-with-a-replica-canoe

Testing ancient Paleolithic migration with a replica canoe

(Left) GPS tracking and modeling of ocean currents toward the end of the experimental voyage. (Right) The team on the water around the time of the left image.

(Left) GPS tracking and modeling of ocean currents toward the end of the experimental voyage. (Right) The team on the water around the time of the left image. Credit: Kaifu et al., 2025/CC-By-ND

At the 30-hour mark, the captain ordered the entire crew to rest, letting the dugout drift freely for a while, which fortunately brought them closer to Yonaguni Island. At hour 40, the island’s silhouette was visible, and over the next five hours, the crew was able to navigate the strong tidal flow along the coast until they reached their landing site: Nama Beach. So the experimental voyage was a success, augmented by the numerical simulations to demonstrate that the boat could make similar voyages from different departure points across both modern and late-Pleistocene oceans.

Granted, it was not possible to recreate Paleolithic conditions perfectly on a modern ocean. The crew first spotted the island because of its artificial lights, although by that time, they were on track navigationally. They were also accompanied by escort ships to ensure the crew’s safety, supplying fresh water twice during the voyage. But the escort ships did not aid with navigation or the dugout captain’s decision-making, and the authors believe that any effects were likely minimal. The biggest difference was the paddlers’ basic modern knowledge of local geography, which helped them develop a navigation plan—an unavoidable anachronism, although the crew did not rely on compasses, GPS, or watches during the voyage.

“Scientists try to reconstruct the processes of past human migrations, but it is often difficult to examine how challenging they really were,” said Kaifu. “One important message from the whole project was that our Paleolithic ancestors were real challengers. Like us today, they had to undertake strategic challenges to advance. For example, the ancient Polynesian people had no maps, but they could travel almost the entire Pacific. There are a variety of signs on the ocean to know the right direction, such as visible land masses, heavenly bodies, swells and winds. We learned parts of such techniques ourselves along the way.”

DOI: “Traversing the Kuroshio: Paleolithic migration across one of the world’s strongest ocean currents,” Science Advances, 2025. 10.1126/sciadv.adv5508  (About DOIs).

DOI: “Palaeolithic seafaring in East Asia: an experimental test of the dugout canoe hypothesis,” Science Advances, 2025. 10.1126/sciadv.adv5507  (About DOIs).

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We’ve had a Denisovan skull since the 1930s—only nobody knew


It’s a Denisovan? Always has been.

After years of mystery, we now know what at least one Denisovan looked like.

A 146,000-year-old skull from Harbin, China, belongs to a Denisovan, according to a recent study of proteins preserved inside the ancient bone. The paleoanthropologists who studied the Harbin skull in 2021 declared it a new (to us) species, Homo longi. But the Harbin skull still contains enough of its original proteins to tell a different story: A few of them matched specific proteins from Denisovan bones and teeth, as encoded in Denisovan DNA.

So Homo longi was a Denisovan all along, and thanks to the remarkably well-preserved skull, we finally know what the enigmatic Denisovans actually looked like.

Two early-human skulls against a black background.

Credit: Ni et al. 2021

The Harbin skull (left) and the Dali skull (right).

Unmasking Dragon Man 

Paleoanthropologist Qiang Ji, of the Chinese Academy of Sciences, and colleagues tried to sequence ancient DNA from several samples of the Harbin skull’s bone and its one remaining tooth, but they had no luck. Proteins tend to be hardier molecules than DNA, though, and in samples from the skull’s temporal bone (the ones on the sides of the head, just behind the cheekbones), the researchers struck pay dirt.

They found fragments of a total of 95 proteins. Four of these had variations that were distinct to the Denisovan lineage, and the Harbin skull matched Denisovans on three of them. That’s enough to confidently say that the Harbin skull had belonged to a Denisovan. So for the past few years, we’ve had images of an almost uncannily well-preserved Denisovan skull—which is a pretty big deal, especially when you consider its complicated history.

While the world is now aware of it, until 2021, only one person had known what the skull looked like since its discovery in the 1930s. It was unearthed in Harbin, in northeast China, during the Japanese occupation of the area. Not wanting it to be seized by the occupying government, the person who found the skull immediately hid it, and he kept it hidden for most of the rest of his life.

He eventually turned it over to scientists in 2018, who published their analysis in 2021. That analysis placed the Harbin skull, along with a number of other fossils from China, in a distinct lineage within our genus, Homo, making them our species’ closest fossil relatives. They called this alleged new species Homo longi, or “Dragon Man.”

The decision to classify Homo longi as a new species was largely due to the skull’s unique combination of features (which we’ll discuss below). But it was a controversial decision, partly because paleoanthropologists don’t entirely agree about whether we should even call Neanderthals a distinct species. If the line between Neanderthals and our species is that blurry, many in the field have questioned whether Homo longi could be considered a distinct species, when it’s even closer to us than the Neanderthals.

Meanwhile, the 2021 paper also left room for debate on whether the skull might actually have belonged to a Denisovan rather than a distinct new species. Its authors acknowledge that one of the fossils they label as Homo longi had already been identified as a Denisovan based on its protein sequences. They also point out that the Harbin skull has rather large molars, which seem to be a common feature in Denisovans.

The paper’s authors argued that their Homo longi should be a separate branch of the hominin lineage, more closely related to us than to Denisovans or Neanderthals. But if the Harbin skull looked so much like Denisovan fossils and so little like fossils from our species, the alleged relationship begins to look pretty dubious. In the end, the 2021 paper’s authors dodged the issue by saying that “new genetic material will test the relationship of these populations to each other and to the Denisovans.”

Which turned out to be exactly what happened.

A ghost lineage comes to life

Denisovans are the ghost in our family tree. For scientists, a “ghost lineage” is one that’s known mostly from genetic evidence, not fossils; like a ghost, it has a presence we can sense but no physical form we can touch. With the extremely well-preserved Harbin skull identified as a Denisovan, though, we’re finally able to look our “ghost” cousins in the face.

Paleogeneticists have recovered Denisovan DNA from tiny fragments of bone and teeth, and even from the soil of a cave floor. Genomics researchers have found segments of Denisovan DNA woven into the genomes of some modern humans, revealing just how close our two species once were. But the handful of Denisovan fossils paleoanthropologists have unearthed are mostly small fragments—a finger bone here, a tooth there, a jawbone someplace else—that don’t reveal much about how Denisovans lived or what they looked like.

We know they existed and that they were something slightly different from Homo sapiens or Neanderthals. We even know when and where they lived and a surprising amount about their genetics, and we have some very strong hints about how they interacted with our species and with Neanderthals. But we didn’t really know what they looked like, and we couldn’t hope to identify their fossils without turning to DNA or protein sequences.

Until now.

Neanderthals and Denisovans probably enjoyed the view from Denisova Cave, too. Credit: loronet / Flickr

The face of a Denisovan

So what did a Denisovan look like? Harbin 1 has a wide, flattish face with small cheekbones, big eye sockets, and a heavy brow. Its upper jaw juts forward just a little, and it had big, robust molars. The cranium itself is longer and less dome-like than ours, but it’s roomy enough for a big brain (about 1,420 millimeters).

Some of those traits, like the large molars and the long, low cranium, resemble those of earlier hominin species such as Homo erectus or Homo heidelbergensis. Others, like a relatively flat face, set beneath the cranium instead of sticking out in front of it, look more like us. (Early hominins, like Australopithecus afarensis, don’t really have foreheads because their skulls are arranged so their brains are right behind their faces instead of partly above them, like ours.)

In other words, Harbin’s features are what paleoanthropologists call a mosaic, with some traits that look like they come from older lineages and some that seem more modern. Mosaics are common in the hominin family tree.

But for all the detail it reveals about the Denisovans, Harbin is still just one skull from one individual. Imagine trying to reconstruct all the diversity of human faces from just one skull. We have to assume that Densiovans—a species that spanned a huge swath of our planet, from Siberia to Taiwan, and a wide range of environments, from high-altitude plateaus in Tibet to subtropical forests—were also a pretty diverse species.

It’s also worth remembering that the Harbin skull is exactly that: a skull. It can’t tell us much about how tall its former user was, how they were built, or how they moved or worked during their life. We can’t even say for sure whether Harbin is osteologically or genetically male or female. In other words, some of the mystery of the Denisovans still endures.

What’s next?

In the 2021 papers, the researchers noted that the Harbin skull also bears a resemblance to a 200,000- to 260,000-year-old skull found in Dali County in northwestern China, a roughly 300,000-year-old skull found in Hualong Cave in eastern China, and a 260,000-year-old skull from Jinniushi (sometimes spelled Jinniushan) Cave in China. And some fossils from Taiwan and northern China have molars that look an awful lot like those in that Tibetan jawbone.

“These hominins potentially also belong to Denisovan populations,” write Ji and colleagues. That means we might already have a better sample of Denisovan diversity than this one skull suggests.

And, like the Harbin skull, the bones and teeth of those other fossils may hold ancient DNA or proteins that could help confirm that intriguing possibility.

Science, 2023 DOI: 10.1126/science.adu9677 (About DOIs).

Photo of Kiona N. Smith

Kiona is a freelance science journalist and resident archaeology nerd at Ars Technica.

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skull-long-thought-to-be-cleopatra’s-sister’s-was-actually-a-young-boy

Skull long thought to be Cleopatra’s sister’s was actually a young boy

Scientists have demonstrated that an ancient human skull excavated from a tomb at Ephesos was not that of Arsinoë IV, half-sister to Cleopatra VII. Rather, it’s the skull of a young male between the ages of 11 and 14 from Italy or Sardinia, who may have suffered from one or more developmental disorders, according to a new paper published in the journal Scientific Reports. Arsinoë IV’s remains are thus still missing.

Arsinoë IV led quite an adventurous short life. She was either the third or fourth daughter of Ptolemy XII, who left the throne to Cleopatra and his son, Ptolemy XIII, to rule together. Ptolemy XIII didn’t care for this decision and dethroned Cleopatra in a civil war—until Julius Caesar intervened to enforce their father’s original plan of co-rulership. As for Arsinoë, Caesar returned Cyprus to Egyptian rule and named her and her youngest brother (Ptolemy XIV) co-rulers. This time, it was Arsinoë who rebelled, taking command of the Egyptian army and declaring herself queen.

She was fairly successful at first in battling the Romans, conducting a siege against Alexandria and Cleopatra, until her disillusioned officers decided they’d had enough and secretly negotiated with Caesar to turn her over to him. Caesar agreed, and after a bit of public humiliation, he granted Arsinoë sanctuary in the temple of Artemis in Ephesus. She lived in relative peace for a few years, until Cleopatra and Mark Antony ordered her execution on the steps of the temple—a scandalous violation of the temple as a place of sanctuary. Historians disagree about Arsinoë’s age when she died: Estimates range from 22 to 27.

Archaeologists have been excavating the ancient city of Ephesus for more than a century. The Octagon was uncovered in 1904, and the burial chamber was opened in 1929. That’s where Joseph Keil found a skeleton in a sarcophagus filled with water, but for some reason, Keil only removed the cranium from the tomb before sealing it back up. He took the skull with him to Germany and declared it belonged to a likely female around 20 years old, although he provided no hard data to support that conclusion.

It was Hilke Thur of the Austrian Academy of Sciences who first speculated that the skull may have belonged to Arsinoë IV, despite the lack of an inscription (or even any grave goods) on the tomb where it was found. Old notes and photographs, as well as craniometry, served as the only evidence. The skull accompanied Keil to his new position at the University of Vienna, and there was one 1953 paper reporting on craniometric measurements, but after that, the skull languished in relative obscurity. Archaeologists at the University of Graz rediscovered the skull in Vienna in 2022. The rest of the skeleton remained buried until the chamber was reopened and explored further in the 1980s and 1990s, but it was no longer in the sarcophagus.

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studies-pin-down-exactly-when-humans-and-neanderthals-swapped-dna

Studies pin down exactly when humans and Neanderthals swapped DNA


We may owe our tiny sliver of Neanderthal DNA to just a couple of hundred Neanderthals.

The artist’s illustration shows what the six people buried at the Ranis site, who lived between 49, 500 and 41,000 years ago, may have looked like. Two of these people are mother and daughter, and the mother is a distant cousin (or perhaps a great-great-grandparent or great-great-grandchild) to a woman whose skull was found 130 kilometers away in what’s now Czechia. Credit: Sumer et al. 2024

Two recent studies suggest that the gene flow (as the young people call it these days) between Neanderthals and our species happened during a short period sometime between 50,000 and 43,500 years ago. The studies, which share several co-authors, suggest that our torrid history with Neanderthals may have been shorter than we thought.

Pinpointing exactly when Neanderthals met H. sapiens  

Max Planck Institute of Evolutionary Anthropology scientist Leonardo Iasi and his colleagues examined the genomes of 59 people who lived in Europe between 45,000 and 2,200 years ago, plus those of 275 modern people whose ancestors hailed from all over the world. The researchers cataloged the segments of Neanderthal DNA in each person’s genome, then compared them to see where those segments appeared and how that changed over time and distance. This revealed how Neanderthal ancestry got passed around as people spread around the world and provided an estimate of when it all started.

“We tried to compare where in the genomes these [Neanderthal segments] occur and if the positions are shared among individuals or if there are many unique segments that you find [in people from different places],” said University of California Berkeley geneticist Priya Moorjani in a recent press conference. “We find the majority of the segments are shared, and that would be consistent with the fact that there was a single gene flow event.”

That event wasn’t quite a one-night stand; in this case, a “gene flow event” is a period of centuries or millennia when Neanderthals and Homo sapiens must have been in close contact (obviously very close, in some cases). Iasi and his colleagues’ results suggest that happened between 50,500 and 43,000 years ago. But it’s quite different from our history with another closely related hominin species, the now-extinct Denisovans, with whom different Homo sapiens groups met and mingled at least twice on our way to taking over the world.

In a second study, Arev Sümer (also of the Max Planck Institute) and her colleagues found something very similar in the genomes of people who lived 49,500 to 41,000 years ago in what’s now the area around Ranis, Germany. The Ranis population, based on how their genomes compare to other ancient and modern people, seem to have been part of one of the first groups to split off from the wave of humans who migrated out of Africa, through the Levant, and into Eurasia sometime around 50,000 years ago. They carried with them traces of what their ancestors had gotten up to during that journey: about 2.9 percent of their genomes were made up of segments of Neanderthal ancestry.

Based on how long the Ranis people’s segments of Neanderthal DNA were (longer chunks of Neanderthal ancestry tend to point to more recent mixing), the interspecies mingling happened about 80 generations, or about 2,300 years, before the Ranis people lived and died. That’s about 49,000 to 45,000 years ago. The dates from both studies line up well with each other and with archaeological evidence that points to when Neanderthal and Homo sapiens cultures overlapped in parts of Europe and Asia.

What’s still not clear is whether that period of contact lasted the full 5,000 to 7,000 years, or if, as Johannes Krause (also of the Max Planck Institute) suggests, it was only a few centuries—1,500 years at the most—that fell somewhere within that range of dates.

Artist’s depiction of a Neanderthal.

Natural selection worked fast on our borrowed Neanderthal DNA

Once those first Homo sapiens in Eurasia had acquired their souvenir Neanderthal genes (forget stealing a partner’s hoodie; just take some useful segments of their genome), natural selection got to work on them very quickly, discarding some and passing along others, so that by about 100 generations after the “event,” the pattern of Neanderthal DNA segments in people’s genomes looked a lot like it does today.

Iasi and his colleagues looked through their catalog of genomes for sections that contained more (or less) Neanderthal ancestry than you’d expect to find by random chance—a pattern that suggests that natural selection has been at work on those segments. Some of the segments that tended to include more Neanderthal gene variants included areas related to skin pigmentation, the immune response, and metabolism. And that makes perfect sense, according to Iasi.

“Neanderthals had lived in Europe, or outside of Africa, for thousands of years already, so they were probably adapted to their environment, climate, and pathogens,” said Iasi during the press conference. Homo sapiens were facing selective pressure to adapt to the same challenges, so genes that gave them an advantage would have been more likely to get passed along, while unhelpful ones would have been quick to get weeded out.

The most interesting questions remain unanswered

The Neanderthal DNA that many people carry today, the researchers argue, is a legacy from just 100 or 200 Neanderthals.

“The effective population size of modern humans outside Africa was about 5,000,” said Krause in the press conference. “And we have a ratio of about 50 to 1 in terms of admixture [meaning that Neanderthal segments account for about 2 percent of modern genomes in people who aren’t of African ancestry], so we have to say it was about 100 to maybe 200 Neanderthals roughly that mixed into the population.” Assuming Krause is right about that and about how long the two species stayed in contact, a Homo sapiens/Neanderthal pairing would have happened every few years.

So we know that Neanderthals and members of our species lived in close proximity and occasionally produced children for at least several centuries, but no artifacts, bones, or ancient DNA have yet revealed much of what that time, or that relationship, was actually like for either group of people.

The snippets of Neanderthal ancestry left in many modern genomes, and those of people who lived tens of thousands of years ago, don’t offer any hints about whether that handful of Neanderthal ancestors were mostly male or mostly female, which is something that could shed light on the cultural rules around such pairings. And nothing archaeologists have unearthed so far can tell us whether those pairings were consensual, whether they were long-term relationships or hasty flings, or whether they involved social relationships recognized by one (or both) groups. We may never have answers to those questions.

And where did it all happen? Archaeologists haven’t yet found a cave wall inscribed with “Og heart Grag,” but based on the timing, Neanderthals and Homo sapiens probably met and lived alongside each other for at least a few centuries, somewhere in “the Near East,” which includes parts of North Africa, the Levant, what’s now Turkey, and what was once Mesopotamia. That’s one of the key routes that people would have followed as they migrated from Africa into Europe and Asia, and the timing lines up with when we know that both Homo sapiens and Neanderthals were in the area.

“This [same] genetic admixture also appears in East Asia and Australia and the Americas and Europe,” said Krause. “If it would have happened in Europe or somewhere else, then the distribution would probably look different than what we see.”

Science, 2023 DOI: 10.1126/science.adq3010;

Nature, 2023 DOI: 10.1038/s41586-024-08420-x;

(About DOIs).

Photo of Kiona N. Smith

Kiona is a freelance science journalist and resident archaeology nerd at Ars Technica.

Studies pin down exactly when humans and Neanderthals swapped DNA Read More »

paleolithic-deep-cave-compound-likely-used-for-rituals

Paleolithic deep-cave compound likely used for rituals

Archaeologists excavating a paleolithic cave site in Galilee, Israel, have found evidence that a deep-cave compound at the site may have been used for ritualistic gatherings, according to a new paper published in the Proceedings of the National Academy of Sciences (PNAS). That evidence includes the presence of a symbolically carved boulder in a prominent placement, and well as the remains of what may have been torches used to light the interior. And the acoustics would have been conducive to communal gatherings.

Dating back to the Early Upper Paleolithic period, Manot Cave was found accidentally when a bulldozer broke open its roof during construction in 2008. Archaeologists soon swooped in and recovered such artifacts as stone tools, bits of charcoal, remains of various animals, and a nearly complete human skull.

The latter proved to be especially significant, as subsequent analysis showed that the skull (dubbed Manot 1) had both Neanderthal and modern features and was estimated to be about 54,700 years old. That lent support to the hypothesis that modern humans co-existed and possibly interbred with Neanderthals during a crucial transition period in the region, further bolstered by genome sequencing.

The Manot Cave features an 80-meter-long hall connecting to two lower chambers from the north and south. The living section is near the entrance and was a hub for activities like flint-knapping, butchering animals, eating, and other aspects of daily life. But about eight stories below, there is a large cavern consisting of a high gallery and an adjoining smaller “hidden” chamber separated from the main area by a cluster of mineral deposits called speleothems.

That’s the area that is the subject of the new PNAS paper. Unlike the main living section, the authors found no evidence of daily human activities in this compound, suggesting it served another purpose—most likely ritual gatherings.

Paleolithic deep-cave compound likely used for rituals Read More »

study:-why-aztec-“death-whistles”-sound-like-human-screams

Study: Why Aztec “death whistles” sound like human screams

Aztec death whistles don’t fit into any existing Western classification for wind instruments; they seem to be a unique kind of “air spring” whistle, based on CT scans of some of the artifacts. Sascha Frühholz, a cognitive and affective neuroscientist at the University of Zürich, and several colleagues wanted to learn more about the physical mechanisms behind the whistle’s distinctive sound, as well as how humans perceive said sound—a field known as psychoacoustics. “The whistles have a very unique construction, and we don’t know of any comparable musical instrument from other pre-Columbian cultures or from other historical and contemporary contexts,” said Frühholz.

A symbolic sound?

Human sacrifice with original skull whistle (small red box and enlarged rotated view in lower right) discovered 1987–89 at the Ehecatl-Quetzalcoatl temple in Mexico City, Mexico.

Human sacrifice with original skull whistle (small red box and enlarged rotated view in lower right) discovered 1987–89 at the Ehecatl-Quetzalcoatl temple in Mexico City. Credit: Salvador Guillien Arroyo, Proyecto Tlatelolco

For their acoustic analysis, Frühholz et al. obtained sound recordings from two Aztec skull whistles excavated from Tlatelolco, as well as from three noise whistles (part of Aztec fire snake incense ladles). They took CT scans of whistles in the collection of the Ethnological Museum in Berlin, enabling them to create both 3D digital reconstructions and physical clay replicas. They were also able to acquire three additional artisanal clay whistles for experimental purposes.

Human participants then blew into the replicas with low-, medium-, and high-intensity air pressure, and the ensuing sounds were recorded. Those recordings were compared to existing databases of a broad range of sounds: animals, natural soundscapes, water sounds, urban noise, synthetic sounds (as for computers, pinball machines, printers, etc.), and various ancient instruments, among other samples. Finally, a group of 70 human listeners rated a random selection of sounds from a collection of over 2,500 samples.

The CT scans showed that skull whistles have an internal tube-like air duct with a constricted passage, a counter pressure chamber, a collision chamber, and a bell cavity. The unusual construction suggests that the basic principle at play is the Venturi effect, in which air (or a generic fluid) speeds up as it flows through a constricted passage, thereby reducing the pressure. “At high playing intensities and air speeds, this leads to acoustic distortions and to a rough and piercing sound character that seems uniquely produced by the skull whistles,” the authors wrote.

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old-easter-island-genomes-show-no-sign-of-a-population-collapse

Old Easter Island genomes show no sign of a population collapse

A row of grey rock sculptures of human torsos and heads, arranged in a long line.

Rapa Nui, often referred to as Easter Island, is one of the most remote populated islands in the world. It’s so distant that Europeans didn’t stumble onto it until centuries after they had started exploring the Pacific. When they arrived, though, they found that the relatively small island supported a population of thousands, one that had built imposing monumental statues called moai. Arguments over how this population got there and what happened once it did have gone on ever since.

Some of these arguments, such as the idea that the island’s indigenous people had traveled there from South America, have since been put to rest. Genomes from people native to the island show that its original population was part of the Polynesian expansion across the Pacific. But others, such as the role of ecological collapse in limiting the island’s population and altering its culture, continue to be debated.

Researchers have now obtained genome sequence from the remains of 15 Rapa Nui natives who predate European contact. And they indicate that the population of the island appears to have grown slowly and steadily, without any sign of a bottleneck that could be associated with an ecological collapse. And roughly 10 percent of the genomes appear to have a Native American source that likely dates from roughly the same time that the island was settled.

Out of the museum

The remains that provided these genomes weren’t found on Rapa Nui, at least not recently. Instead, they reside at the Muséum National d’Histoire Naturelle in France, having been obtained at some uncertain point in the past. Their presence there is a point of contention for the indigenous people of Rapa Nui, but the researchers behind the new work had the cooperation of the islanders in this project, having worked with them extensively. The researchers’ description of these interactions could be viewed as a model for how this sort of work should be done:

Throughout the course of the study, we met with representatives of the Rapanui community on the island, the Comisión de Desarrollo Rapa Nui and the Comisión Asesora de Monumentos Nacionales, where we presented our research goals and ongoing results. Both commissions voted in favor of us continuing with the research… We presented the research project in public talks, a short video and radio interviews on the island, giving us the opportunity to inquire about the questions that are most relevant to the Rapanui community. These discussions have informed the research topics we investigated in this work.

Given the questionable record-keeping at various points in the past, one of the goals of this work was simply to determine whether these remains truly had originated on Rapa Nui. That was unambiguously true. All comparisons with genomes of modern populations show that all 15 of these genomes have a Polynesian origin and are most closely related to modern residents of Rapa Nui. “The confirmation of the origin of these individuals through genomic analyses will inform repatriation efforts led by the Rapa Nui Repatriation Program (Ka Haka Hoki Mai Te Mana Tupuna),” the authors suggest.

A second question was whether the remains predate European contact. The researchers attempted to perform carbon dating, but it produced dates that made no sense. Some of the remains had dates that were potentially after they had been collected, according to museum records. And all of them were from the 1800s, well after European contact and introduced diseases had shrunk the native population and mixed in DNA from non-Polynesians. Yet none of the genomes showed more than one percent European ancestry, a fraction low enough to be ascribed to a spurious statistical fluke.

So the precise date these individuals lived is uncertain. But the genetic data clearly indicates that they were born prior to the arrival of Europeans. They can therefore tell us about what the population was experiencing in the period between Rapa Nui’s settlement and the arrival of colonial powers.

Back from the Americas

While these genomes showed no sign of European ancestry, they were not fully Polynesian. Instead, roughly 10 percent of the genome appeared to be derived from a Native American population. This is the highest percentage seen in any Polynesian population, including some that show hints of Native American contact that dates to before Europeans arrived on the scene.

Isolating these DNA sequences and comparing them to populations from across the world showed that the group most closely related to the one who contributed to the Rapa Nui population presently resides in the central Andes region of South America. That’s in contrast to the earlier results, which suggested the contact was with populations further north in South America.

Old Easter Island genomes show no sign of a population collapse Read More »

study:-scribes-in-ancient-egypt-had-really-poor-posture-during-work

Study: Scribes in ancient Egypt had really poor posture during work

a scribe’s life —

There were degenerative joint changes in the spines, shoulders, knees, hips, and ankles.

Statues depicting the high dignitary Nefer and his wife (Abusir, Egypt).

Enlarge / Statues depicting the high dignitary Nefer and his wife (Abusir, Egypt).

Martin Frouz/Czech Institute of Egyptology/Charles University.

Repetitive stress injuries are a common feature of modern life, especially for office workers who spend a good chunk of their working days at a desk typing on a computer. Apparently, scribes in ancient Egypt suffered from their own distinctive repetitive stress injuries, according to a new paper published in the journal Scientific Reports that provides fresh insights into how these scribes lived and worked during the third millennium BCE.

Egyptian kings, royal family members, and other elite people from this Fifth Dynasty era were buried in tombs in the acropolis at Abusir rather than at neighboring Giza, which by then had largely filled up thanks to all the activity during the Fourth Dynasty. The Czech Institute of Egyptology at Charles University in Prague has been conducting research at the site since 1960, leading to the discovery of nearly 200 tombs dating back to the Old Kingdom (between 2700 and 2180 BCE). The first human skeletons were excavated in 1976, and there are currently 221 Old Kingdom skeletons in the collection, 102 of which are male.

Scientists started looking into the health status and markers for specific activities in 2009, but it wasn’t until quite recently that there were enough skeletons to conduct a comprehensive study. That’s what Petra Brukner Havelková of Charles University and the National Museum in Prague, Czech Republic, and colleagues set out to do, analyzing the remains of 69 adult males of different social status and different ages at which they died.

The results show the scribes and the reference group differed in just under 4 percent of the various evaluated skeletal traits, which the authors attribute to the overall similarities in the sample (male, same age distribution, no physically demanding activities). However, the individuals identified as scribes had more degenerative joint issues clustered in several well-defined regions compared to males from other occupations, including the joint connecting the lower jaw to the skull, the right collarbone, where the right humerus meets the shoulder, the right thumb’s first metacarpal bone, where the thigh meets the knee, and all along the spine (but especially at the top). These bone changes can be indicators of repetitive stress.

Bad ergonomics?

  • Working positions of scribes. (A) cross-legged (sartorial) position, (B) kneeling-squatting position, (C) standing position. (D) Different position of the legs when sitting.

    Martin Frouz/Jolana Malátková

  • Osteoarthritis of the temporomandibular joint of a supposed family member of Khemetnu, the presumed owner of family tomb AS 79.

    Šárka Bejdová

  • Drawing indicating the most affected regions of the skeletons of scribes with higher prevalence of changes compared to reference group.

    olana Malátková

For instance, the osteoarthritis in the jaw joints may have been caused by the rush stems the scribes used to write hieroglyphics. The scribes would chew on the ends to make a brush, and whenever a pen started getting ragged or too clogged with ink, they would cut off the end and chew the next section to make a new brush.

Most scribes likely wrote with their right hands and used their left to roll papyrus into cylindrical scrolls. Writing with a rush pen required considerable dexterity, and as anyone with carpal tunnel syndrome could tell you, these sorts of repetitive motions can cause excessive stress in the hands and wrists. There were only minor wrist differences between scribes and the control group, but the significant right thumb degeneration in scribes likely corresponds to specific frequently used thumb motions and positions—probably the act of repeatedly pinching their pens, although the authors say more research is needed to make a definitive determination.

The degenerative signs noted in the cervical spines are likely due to the scribes’ typical working position. “The head had to be forward and the spine flexed, changing the center of gravity of the head and putting stress on the spine,” the authors wrote—a posture common to many modern occupations. Prolonged stretches of sitting cross-legged could also have contributed to the observed damage to the cervical spines. There were signs of stresses to the right rotator cuffs, which usually occurs when the arm is in an extended elevated position and is common among painters, for example. It’s also common in people prone to sitting for a long time and typing with unsupported arms.

As for the degenerative changes noted in the knees, hips, and ankles, the authors suggest this indicates that scribes may have sat with the left leg in a kneeling or cross-legged position and the right leg pointed upward—more of a squat or a crouch. Iconography and statues from that era frequently depict scribes in such positions, as well as standing. The authors concluded that scribes probably alternated their arm and leg positions, but the head and cervical spine were always in that stress-inducing forward position.

Where’s a good ergonomic office chair when you need one?

DOI: Scientific Reports, 2024. 10.1038/s41598-024-63549-z  (About DOIs).

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we-now-have-even-more-evidence-against-the-“ecocide”-theory-of-easter-island

We now have even more evidence against the “ecocide” theory of Easter Island

counting rock gardens —

AI analysis of satellite imagery data is a new method for estimating population size.

statues on easter island arranged in a horizontal row

Enlarge / New research lends further credence to the “population crash” theory about Easter Island being just a myth.

Arian Zwegers/CC BY 2.0

For centuries, Western scholars have touted the fate of the native population on Easter Island (Rapa Nui) as a case study in the devastating cost of environmentally unsustainable living. The story goes that the people on the remote island chopped down all the trees to build massive stone statues, triggering a population collapse. Their numbers were further depleted when Europeans discovered the island and brought foreign diseases, among other factors. But an alternative narrative began to emerge in the 21st century that the earliest inhabitants actually lived quite sustainably until that point. A new paper published in the journal Science Advances offers another key piece of evidence in support of that alternative hypothesis.

As previously reported, Easter Island is famous for its giant monumental statues, called moai, built some 800 years ago and typically mounted on platforms called ahu. Scholars have puzzled over the moai on Easter Island for decades, pondering their cultural significance, as well as how a Stone Age culture managed to carve and transport statues weighing as much as 92 tons. The first Europeans arrived in the 17th century and found only a few thousand inhabitants on a tiny island (just 14 by 7 miles across) thousands of miles away from any other land. Since then, in order to explain the presence of so many moai, the assumption has been that the island was once home to tens of thousands of people.

But perhaps they didn’t need tens of thousands of people to accomplish that feat. Back in 2012, Carl Lipo of Binghamton University and Terry Hunt of the University of Arizona showed that you could transport a 10-foot, 5-ton moai a few hundred yards with just 18 people and three strong ropes by employing a rocking motion. In 2018, Lipo proposed an intriguing hypothesis for how the islanders placed red hats on top of some moai; those can weigh up to 13 tons. He suggested the inhabitants used ropes to roll the hats up a ramp. Lipo and his team later concluded (based on quantitative spatial modeling) that the islanders likely chose the statues’ locations based on the availability of fresh water sources, per a 2019 paper in PLOS One.

In 2020, Lipo and his team turned their attention to establishing a better chronology of human occupation of Rapa Nui. While it’s generally agreed that people arrived in Eastern Polynesia and on Rapa Nui sometime in the late 12th century or early 13th century, we don’t really know very much about the timing and tempo of events related to ahu construction and moai transport in particular. In his bestselling 2005 book Collapse, Jared Diamond offered the societal collapse of Easter Island (aka Rapa Nui), around 1600, as a cautionary tale. Diamond controversially argued that the destruction of the island’s ecological environment triggered a downward spiral of internal warfare, population decline, and cannibalism, resulting in an eventual breakdown of social and political structures.

Challenging a narrative

Lipo has long challenged that narrative, arguing as far back as 2007 against the “ecocide” theory. He and Hunt published a paper that year noting the lack of evidence of any warfare on Easter Island compared to other Polynesian islands. There are no known fortifications, and the obsidian tools found were clearly used for agriculture. Nor is there much evidence of violence among skeletal remains. He and Hunt concluded that the people of Rapa Nui continued to thrive well after 1600, which would warrant a rethinking of the popular narrative that the island was destitute when Europeans arrived in 1722.

For their 2020 study, the team applied a Bayesian model-based method to existing radiocarbon dates collected from prior excavations at 11 different sites with ahu. That work met with some mixed opinions from Lipo’s fellow archaeologists, with some suggesting that his team cherry-picked its radiocarbon dating—an allegation he dismissed at the time as “simply baloney and misinformed thinking.” They filtered their radiocarbon samples to just those they were confident related to human occupation and human-related events, meaning they analyzed a smaller subset of all the available ages—not an unusual strategy to eliminate bias due to issues with old carbon—and the results for colonization estimates were about the same as before.

Binghamton University's Robert J. DiNapoli stands next to a rock garden on Rapa Nui, or Easter Island.

Enlarge / Binghamton University’s Robert J. DiNapoli stands next to a rock garden on Rapa Nui, or Easter Island.

Robert J. DiNapoli

The model also integrated the order and position of the island’s distinctive architecture, as well as ethnohistoric accounts, thereby quantifying the onset of monument construction, the rate at which it occurred, and when it likely ended. This allowed the researchers to test Diamond’s “collapse” hypothesis by building a more precise timeline of when construction took place at each of the sites. The results demonstrated a lack of evidence for a pre-contact collapse and instead offered strong support for a new emerging model of resilient communities that continued their long-term traditions despite the impacts of European arrival.

Fresh evidence

Now Lipo is back with fresh findings in support of his alternative theory, having analyzed the landscape to identify all the agricultural areas on the island. “We really wanted to look at the evidence for whether the island could in fact support such a large number of people,” he said during a media briefing. “What we know about the pre-contact people living on the island is that they survived on a combination of marine resources—fishing accounted for about 50 percent of their diet—and growing crops,” particularly the sweet potato, as well as taro and yams.

He and his co-authors set out to determine how much food could be produced agriculturally, extrapolating from that the size of a sustainable population. The volcanic soil on Easter Island is highly weathered and thus poor in nutrients essential for plant growth: nitrogen, phosphorus and potassium primarily, but also calcium, magnesium, and sulfur. To increase yields, the natives initially cut down the island’s trees to get nutrients back into the soil.

When there were no more trees, they engaged in a practice called “lithic mulching,” a form of rock gardening in which broken rocks were added to the first 20 to 25 centimeters (about 8 to 10 inches) of soil. This added essential nutrients back into the soil. “We do it ourselves with non-organic fertilizer,” said Lipo. “Essentially we use machines to crush rock into tiny pieces, which is effective because it exposes a lot of surface area. The people in Rapa Nui are doing it by hand, literally breaking up rocks and sticking them in dirt.”

There had been only one 2013 study aimed at determining the island’s rock-garden capacity, which relied on near-infrared bands from satellite images. The authors of that study estimated that between 4.9 and 21.2 km2 of the island’s total area comprised rock gardens, although they acknowledged this was likely an inaccurate estimation.

A map of results from the analysis of rock gardens on Easter Island.

Enlarge / A map of results from the analysis of rock gardens on Easter Island.

Carl Lipo

Lipo et al. examined satellite imagery data collected over the last five years, not just in the near-infrared, but also short-wave infrared (SWIR) and other visible spectra. SWIR is particularly sensitive to detecting water and nitrogen levels, making it easier to pinpoint areas where lithic mulching occurred. They trained machine-learning models on archaeological field identifications of rock garden features to analyze the SWIR data for a new estimation of capacity.

The result: Lipo et al. determined that the prevalence of rock gardening was about one-fifth of even the most conservative previous estimates of population size on Easter Island. They estimate that the island could support about 3,000 people—roughly the same number of inhabitants European explorers encountered when they arrived. “Previous studies had estimated that the island was fairly covered with mulch gardening, which led to estimates of up to 16,000 people,” said Lipo. “We’re saying that the island could never have supported 16,000 people; it didn’t have the productivity to do so. This pre-European collapse narrative simply has no basis in the archaeological record.”

“We don’t see demographic change decline in populations prior to Europeans’ arrival,” Lipo said. “All the [cumulative] evidence to date shows a continuous growth until some plateau is reached. It certainly was never an easy place to live, but people were able to figure out a means of doing so and lived within the boundaries of the capacity of the island up until European arrival.” So rather than being a cautionary tale, “Easter Island is a great case of how populations adapt to limited resources on a finite place, and do so sustainably.”

DOI: Science Advances, 2024. 10.1126/sciadv.ado1459  (About DOIs).

Binghamton University archaeologist Carl Lipo has shed light on some of the ancient mysteries of Easter Island (Rapa Nui) through his ongoing research. Credit: Binghamton University, State University of New York

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bodies-found-in-neolithic-pit-were-likely-victims-of-ritualistic-murder

Bodies found in Neolithic pit were likely victims of ritualistic murder

murder most foul —

One victim may have been hogtied alive in pit, à la Mafia-style ligature strangulation.

View taken from the upper part of the 255 storage pit showing the three skeletons, with one individual in a central position

Enlarge / Three female skeletons found in a Neolithic storage pit in France show signs of ritualistic human sacrifice.

. Beeching/Ludes et al., 2024

Archaeologists have discovered the remains of two women in a Neolithic tomb in France, with the positioning of the bodies suggesting they may have been ritualistically murdered by asphyxia or self-strangulation, according to a recent paper published in the journal Science Advances.

(WARNING: graphic descriptions below.)

France’s Rhône Valley is home to several archaeological sites dating to the end of the Middle Neolithic period (between 4250 and 3600/3500 BCE in the region); the sites include various storage silos, broken grindstones, imported ceramics, animal remains (both from communal meals and sacrifices), and human remains deposited in sepulchral pits. Saint-Paul-Trois-Châteaux is one such site.

According to Bertrand Ludes of the Université Paris Cité and his co-authors, the remains of the three women were found in Pit 69, a structure aligned with the summer and winter solstices, as was often the case in ancient agrarian societies. But the body positioning was decidedly atypical. One woman (No. 1), around age 50, was in the center of the pit, reclining on her side with a vase near her head. The other two bodies were just beneath an overhang. Woman No. 2 was on her back, legs bent, with a piece of grindstone placed on her skull. Woman No. 3 was in a prone position, knees bent, with her neck on the thorax of Woman No. 2 and two chunks of grindstone placed on her back.

The unusual positions imply a forceful, deliberate placement, according to the authors, suggesting they died in the pit rather than being tossed in after death. As for the cause of death, the archaeological evidence combined with recent forensic studies suggests “homicidal ligature strangulation” and “forced positional asphyxia”—at least for two of the three women. Woman No. 2, for example, would have struggled to breathe on her back, especially with the weight of Woman No. 3 pressing down on her neck (positional asphyxia), further exacerbated by the placement of the grindstone fragment.

Saint-Paul-Trois-Châteaux and the area surrounding pits 69 and 70.

Enlarge / Saint-Paul-Trois-Châteaux and the area surrounding pits 69 and 70.

Ludes et al., Sci. Adv. 10, eadl3374 (2024)

Woman No. 3’s prone position would mean she also would have struggled to breathe, and the volume of blood pumped by the heart would have sharply decreased, leading to cardiac arrest, a form of positional asphyxia now known as “prone restraint cardiac arrest.” All these clinical terms don’t quite capture the horrifically cruel nature of the manner of death. Given the placement of the woman’s knees—bent at more than a 90-degree angle, making the legs almost vertical—it’s possible she was tied up lying on her stomach, with the ligature connecting her ankles to her neck (similar to being hog-tied). In this position, “self-strangulation becomes inevitable,” the authors wrote, particularly if the mouth and nose are obstructed or there is cervical compression—say, from the strategic placement of grindstone fragments.

This form of torture, known as incaprettamento, has been used by the Italian Mafia, per Ludes et al., often to punish traitors, and dates as far back as the Italian Mesolithic era, “suggesting a highly ancient origin within ceremonial sites.” Sometimes, the victim would be strangled and the body tied up postmortem before the remains were disposed of. Ludes et al. believe Woman No. 3 would probably have been placed in the burial pit and tied up while still alive before self-strangling to death.

  • Reconstruction of the remains, blocked under the overhang of the wall of the storage pit lined with straw.

    Ludes et al., Sci. Adv. 10, eadl3374 (2024)

  • Reconstruction of skeletal remains for Woman No. 2 positioned on her back with bent knees.

    Ludes et al., Sci. Adv. 10, eadl3374 (2024)

  • Reconstruction of skeletal remains for Woman No. 3 in a prone position on her stomach with bent knees. The feet are behind the pelvis/toward the lower back, and the hands are tied behind the back.

    Ludes et al., Sci. Adv. 10, eadl3374 (2024)

  • Mesolithic rock art scene from the Addaura Cave is believed to depict ritual sacrifice by ligature strangulation (bolded figure).

    B. Ludes et al., 2024

In fact, one scene from Mesolithic rock art found in the Addaura Cave in Sicily, Italy, seems to depict a ritual sacrifice by ligature strangulation. There are 11 human figures and the figure of a slain deer. Nine of the human figures form a circle, within which are the other two human figures (male, judging by the erect genitalia). Those figures are shown lying on their stomachs in a prone position, legs folded beneath them, with a rope stretched between their ankles and necks. The erect male genitalia, and one figure drawn with his tongue hanging out, are both signs of strangulation or hanging, per the authors.

It is notoriously difficult to distinguish between a merely violent death and one with ritualistic overtones when it comes to prehistoric remains. So Ludes et al. combed through existing literature for reports of similar cases. They found 20 cases of probable ligature strangulation or positional asphyxia in total across 14 different archaeological sites in Eastern Europe and Catalonia, spanning nearly 2,000 years. The individuals were found lying on their backs or sides, lower limbs flexed until the feet aligned with the pelvis, indicating hip extension. The oldest remains were found at sites in the Czech Republic and date back to between 5400 and 4800 BCE; the three women found at Saint-Paul-Trois-Châteaux are the most recent.

The latter site in particular has elements that “suggest a profound interconnection between religious systems and power structure in an agricultural society,” the authors concluded—namely, various structures aligned with summer and winter solstices indicative of an agricultural cycle and the placement of two women facing the central woman. It’s unclear why the women were sacrificed, the authors added, but such ritualistic sacrifice likely developed across central and southern Europe sometime in the Mesolithic and evolved over the course of two millennia before culminating in the late Middle Neolithic.

Science Advances, 2024. DOI: 10.1126/sciadv.adl3374  (About DOIs).

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